Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes.
نویسندگان
چکیده
منابع مشابه
Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5'-end carries a phosphate group and the complementary deoxynucleo...
متن کاملEffects of base mismatches on joining of short oligodeoxynucleotides by DNA ligases.
The requirement for Watson-Crick base pairing surrounding a nick in duplex DNA to be sealed by DNA ligase is the basis for oligonucleotide ligation assays that distinguish single base mutations in DNA targets. Experiments in a model system demonstrate that the minimum length of oligonucleotide that can be joined differs for different ligases. Thermus thermophilus (Tth) DNA ligase is unable to j...
متن کامل[Non-homologous DNA end joining].
DNA double strand breaks (DSB) are the most serious form of DNA damage. Repair of DSBs is important to prevent chromosomal fragmentation, translocations and deletions. Non-homologous end joining (NHEJ) is one of three major pathways for the repair of DSBs in human cells. In this process two DNA ends are joined directly, usually with no sequence homology, although in the case of same polarity of...
متن کاملA novel DNA joining activity catalyzed by T4 DNA ligase.
The use of T4 and E. coli DNA ligases in genetic engineering technology is usually associated with nick-closing activity in double stranded DNA or ligation of 'sticky-ends' to produce recombinant DNA molecules. We describe in this communication the ability of T4 DNA ligase to catalyze intramolecular loop formation between annealed oligodeoxyribonucleotides wherein Watson-Crick base pairing is a...
متن کاملOn the Expressiveness of Joining
The expressiveness of communication primitives has been explored in a common framework based on the π-calculus by considering four features: synchronism (asynchronous vs synchronous), arity (monadic vs polyadic data), communication medium (shared dataspaces vs channel-based), and pattern-matching (binding to a name vs testing name equality vs intensionality). Here another dimension coordination...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1968
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.60.1.285